Year: 2016 | Month: April | Volume 9 | Issue 2
Molecular Characterization and virulence pattern studies of rice (Oryza Sativa) blast (Magnaporthe Oryzae) disease
Abstract:
Rice blast, major fungal pathogen attacking rice limits the yield in all major rice growing regions of the world, especially in warm, high humid and dry upland environments. The population genetics of M.oryza were analyzed in three locations i.e. Mandya (Southern dry zone), Ponnampet (Hilly zone) and Bangalore for (pathogenicity test). Disease was evaluated for their pathogenic virulence and genetic characterization using Avr gene specific markers. Isolates were classified into three groups based on pathogenicity viz., seven- severely pathogenic (isolates), 11 moderately pathogenic and three mildly pathogenic isolates. Study revealed, among 21 isolates collected, Avr genes were found in 18 isolates for gene Avr Pita, 15 for Avr-Pitz, four for Avr- Co-39, 12 in Avr-Pia and 8 in Avr- Pik gene. The isolates of Mandya were highly variable than Ponnampet (through cluster analysis). This study helps in understanding the response of different rice accessions to blast in different locations, the distribution and contribution of Avr genes for host plant resistance.
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